Know the difference between an antiseptic vs. How did we identify the index case for this experiment?ġ. Why did we use the organism, Serratia marcescens for this experiment? 5.
What is a propagated transmission epidemic? 4.
What is the part of a bacterial cell that is used to form the structure of a biofilm? 10. Bacteria growing in a biofilm are (more/less) resistant to antibiotics. How did we observe a biofilm in class? 3. Name at least three places where biofilms can form in the human body? 2. What is the Kirby-Bauer test used for? 9. What is the difference between a bactericidal and bacteriostatic antibiotic? 6. Antibiotics that act on the cell wall are more effective against which bacteria? Explain why. Bacteria that are susceptible to the antibiotic will produce an area with no growth surrounding the antibiotic disk on the plate. We created a of bacterial growth on the plate using a cotton swab. What was the name of the agar media we used? 2. Respiration Tests: Catalase Oxidase Nitrate reduction Testing for aerobic or anaerobic respiration? Enzyme Name Reagents added to media What does a positive and negative test result look like? After adding Nitrate A and B to the tube: Red color No color change After adding Nitrate A, B, and C (zinc): Red color = No color change = 1 Additional questions: What gas is produced in a positive test? This enzyme is present in the bacterial cell wall and participates in what part of respiration? What is the purpose of adding the zinc to the nitrate reduction test? what is it doing in the broth? Which gas could be present in the Durham tube if denitrification has occurred?Ĩ. Blackening of the media indicates production of gas. Yellow slant and yellow butt indicate which sugars are being fermented? 5. Red slant and yellow butt indicate which sugars are being fermented? 4. When the products are acidic the indicator changes to the color and when the products are alkaline the indicator changes to the color 3. Which of these three sugars is added in the smallest concentration? 2. The media contains sucrose, glucose, and lactose. The media is chemically (complex/defined) (circle one).ĭelete 7. Are the products produced from a positive test acidic or alkaline? 6. What does a positive and negative result look like? 5. This tests for the presence of the enzyme called 4. Is citrate a good source of energy for most bacteria? 2. What does a positive and negative result look like? 6. Specific compound we are testing for is called 3. Testing for the ability to ferment to produce neutral or non-acidic end products. What does a positive and negative result look like? oboz 5.
What carbohydrate is being fermented in this test? 3. What organism causes strep throat, and what type of hemolysis does it produce?Įnzymes: Starch Plate Exoenzyme testing for? Urea broth Reagent/indicator added to media? What does a positive and negative result look like? Product(s) of hydrolysis? 4. coli or E aerogenes growing on this plate Explain what alpha, beta, and gamma hemolysis looks like on Blood agar. aerogenes growing on this plate Be able to identify E.
SPORE STAIN MAC
What genus of bacteria can this stain identify, and What is the what diseases endospore can they coat made cause? of?ĭifferential/ Selective and Biochemical Tests: MSA MAC Inhibitor EMB Blood Agar Substrate Indicator Media selects for (type of bacteria, and/or genus) NA N/A Color of results for a positive fermentation? Color of results for a negative fermentation? Are the products of fermentation acidic or alkaline? Additional questions: conti N/A How would Staphylococcus aureus appear growing on this plate? Be able to identify E. Transcribed image text: Negative stain Gram stain Acid Fast Spore stain Capsule Stain Purpose of this stain? Primary staining Reagent(s) Primary stain: Mordant: Decolorizer N/A N/A Counterstain NA What should the results look like? Include colors of stained structures, background and cell walls Additional questions: Why do the bacterial cells resist being stained? What is the most critical step of the Gram stain? Why? The cell walls of acid- fast positive bacteria are comprised of what? Endospores are associated with pathogenicity because they are able to withstand Capsules are associated with pathogenicity because they resist (name at least four things).
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